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KMID : 0613820100200091365
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Journal of Life Science
2010 Volume.20 No. 9 p.1365 ~ p.1372
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Effects of Extracts from Dried Yam on Antioxidant and Growth of Human Cancer Cell Lines
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Jang Joo-Ri
Hwang Seong-Yeon
Lim Sun-Young
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Abstract
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We investigated the inhibitory effects of solvent extracts from dried yam on H₂O₂-induced oxidative stress and growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells). Yam (Dioscoreacea) has been recognized as a healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, and anti-mutagenic activities, as well as its ability to decrease blood glucose and cholesterol levels. In order to determine the protective effect on H₂O₂-induced oxidative stress, DCFH-DA (dichlorodihydrofluorescin diacetate) assay was conducted. Acetone with methylene chloride (A+M) extract of dried yam appeared to reduce the levels of intracellular reactive oxygen species (ROS) with dose responses. Among the fractions, 85% aq. methanol fraction showed the highest protective effect on production of lipid peroxides. Inhibitory effects of A+M and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner. The treatments of n-hexane, 85% aq. methanol and n-butanol fractions (? 0.5 ㎎/ml concentrations) significantly inhibited the growth of both cancer cells (p＜0.05). From these results, 85% aq. methanol fraction showed inhibitory effects on cellular oxidation and growth of human cancer cells, suggesting that this fraction may contain active compounds of dried yam.
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KEYWORD
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Dried yam, human cancer cells, growth inhibition, antioxidant, reactive oxygen species
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